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SHIFTING TOPOLOGY OF STEM CELLS AND MOLECULAR EXPRESSION
DURING FEATHER REGENERATIVE CYCLING
by
Syuzanna Avetyan
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(EXPERIMENTAL AND MOLECULAR PATHOLOGY)
December 2008
Copyright 2008 Syuzanna Avetyan
Object Description
| Title | Shifting topology of stem cells and molecular expression during feather regenerative cycling |
| Author | Avetyan, Syuzanna |
| Author email | savetyan@yahoo.com; savetyan@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Experimental & Molecular Pathology |
| School | Keck School of Medicine |
| Date defended/completed | 2008-10-20 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-12-10 |
| Advisor (committee chair) | Chuong, Cheng Ming |
| Advisor (committee member) |
Widelitz, Randall Wu, Ping Wu, Lingtao Bellusci, Saverio Hofman, Florence |
| Abstract | Feather is known for its robust regenerative ability. Stem cells in the feather have recently been mapped. However different parts of the chicken body have different size feathers. How stem cells are managed differently in these different feathers has not been elucidated. Here we analyze the growth pattern of feathers from neck and saddle feather. We analyze the topological arrangement of stem cell, TA cell, differentiated cell during different regenerative feather cycling. We found the stem cell is in a ring configuration during growth phase but shifted down to smaller ring or to assume a Ushape flanking the dermal papilla. The number of stem cells remains rather constant during stem cell cycling but also changes around three folds. On the contrast the number of TA cells change up to twenty thirty fold differences. There also appear to have higher stem cell number and a larger dermal papilla in the saddle compared to the neck feather. We found N-CAM and Tenascin-C expressed in the niche next to the stem cells. We also found TGF-beta2 enriched in the stem cells in the resting phase of the dermal papilla. This work suggests there is a dramatic shifting of stem cell topology during feather cycling, probably to accommodate the different homeotic relationship between stem, TA and differentiated cells. The dynamic changes of molecules also refract the dynamic changes of the microenvironment in the stem cell niche. Further investigation would be required to understand their molecular mechanism and regulation. |
| Keyword | stem cells; transient amplifying cells; differentiated cells |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1902 |
| Contributing entity | University of Southern California |
| Rights | Avetyan, Syuzanna |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Avetyan-2521 |
| Archival file | uscthesesreloadpub_Volume14/etd-Avetyan-2521.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Full text | SHIFTING TOPOLOGY OF STEM CELLS AND MOLECULAR EXPRESSION DURING FEATHER REGENERATIVE CYCLING by Syuzanna Avetyan A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (EXPERIMENTAL AND MOLECULAR PATHOLOGY) December 2008 Copyright 2008 Syuzanna Avetyan |
